ExFLP-24 Determination of Glass Particles in Jam or Jelly
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7745B930BCF7419288CC009ED9E2EA5F |
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0.01 |
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页数： |
3 |
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日期： |
2012-3-3 |
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Published by: POLYSCIENCE PUBLICATIONS, P.O. Box 1606, Station St-Martin, Laval,Quebec, Canada H7V 3P9. TEL.: 1-800-840-5870. FAX: (450) 688-1930.,Government of Canada Gouvernement du Canada,Laboratory Procedure ExFLP-24,April 1996,HEALTH PROTECTION BRANCH,OTTAWA,DETERMINATION OF GLASS PARTICLES IN JAMS OR JELLY,M.A.Rivers,Research Division, Bureau of Microbial Hazards, Food Directorate,Health Canada, Ottawa ON K1A 0L2,1. APPLICATION,This method is applicable to the sampling and examination of jam and jelly for glass particles.,2. DEFINITION OF TERMS,A lot is defined as that amount (volume, weight, etc.) of the food which is produced, stored and/or,shipped under conditions as nearly uniform as possible, preferably designated by a common container,code or marking and, in any event, consisting of not more than one variety, grade, or type of product,from a single identifiable source.,3. COLLECTION OF SAMPLES,3.1 Obtain twenty-four (24) sample units selected at random from the lot. Each sample unit,should consist of a whole jar. Twenty-four (24) sample units constitute a sample.,3.2 Each sample unit must be kept separate and labelled 1,2,3 etc. Complete information,respecting the lot size, size of individual jars, country of origin, exporter, importer or domestic,manufacturer and product and lot identification should be recorded and should accompany the,sample.,4. MATERIALS AND SPECIAL EQUIPMENT,Note: All apparatus in contact with the jam or jelly to be analyzed should not be glass or polyethylene.,Stainless steel apparatus is recommended.,1) Stainless steel beaker (> 2 L). Should be large enough to accommodate the entire contents of,the jar plus 3 rinsings. .,2) Concentrated HCl,3) U.S. standard No. 100 sieve,4) Suction filtration apparatus with Buchner or Hirsch funnel (5-7 cm perforated plate),5) Ruled filter paper - larger than funnel plate,6) Petri dishes to suit size of filter papers used,ExFLP-24,- 2 - April 1996,7) Drying oven (100°C),8) Magnifying lens (3-5x),9) Stereoscopic microscopic (30-70x),10) Polarizing microscopic or compound microscope equipped with polarizing filters,5. PROCEDURE,The examination shall be carried out in accordance with the following instructions:,5.1 Analytical Units,5.1.1 The entire contents of the jar constitutes an analytical unit.,5.2 Isolation,5.2.1 Empty contents of jar into a stainless steel beaker. Retain empty jar and lid.,5.2.2 Examine the jar under magnifying lens (3-5x) for chip damage, especially around the top.,5.2.3 Rinse out the jar and lid 3 times with water and add rinsings to stainless steel beaker.,At this point concntrated HCl (2% v/v) may be added to jams that contain excessive,pulp (e.g. peach, apricot, strawberry, etc.).,5.2.4 Heat the content of the stainless steel beaker to boiling with occasional stirring with a spoon,and boil 5 min.,5.2.5 Transfer slurry quantitatively to a No. 100 sieve and wet-sieve with a forceful stream of hot,(50-70°C) tap water until rinse water runs clear.,5.2.6 Quantitatively transfer residue to the original stainless steel beaker and fill to 1 L with hot tap,water.,5.2.7 Stir contents vigorously for 30 seconds.,5.2.8 Let stand 5 minutes.,5.2.9 Decant suspended food material without disturbing the bottom contents.,5.2.10 If there are numerous seeds remaining as in raspberry and strawberry jams, continue as,follows, otherwise go to Step 5.2.11. Resuspend seeds in approx. 50 mL water. Dry in oven,(100°C) for 3 hrs. Fill container to approx. 1 L with water. Stir vigorously for 30 sec. Let stand,5 min and decant. Repeat if neccessary. Usually only 2 fillings and decantings are,neccessary. Filter bottom contents asin Step 5.2.12.,5.2.11 Repeat filling and decanting until most of the plant tissue is removed. Standing time could be,reduced. It should be no less than that which is required for the plant tissue to become,motionless.,5.2.12 Filter bottom contents onto ruled filter paper.,5.2.13 Repeat isolation for remaining twenty-three (23) analytical units.,5.3 Examination,5.3.1 Examine filter paper microscopically (30x) for glass fragments.,ExFLP-24,- 3 - April 1996,5.3.2 Particles of glass must be confirmed by examining through crossed polars on a polarizing or,compound microscope. Because glass is isotropic to polarized light it should appear dark like,the remainder of all the background.,5.4 Recording Results: ExFLP-24,5.4.1 Record the number and size, and, if possible, the origin of all glass particles.,5.4.2 Record the amount and size of chip damage on the jar.,6. INTERPRETATION,6.1 See Table 2 of “Health Protection Branch Standards and Guidelines for Microbiological Safety,and General Cleanl……
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